Services
The ETH Zurich Flow Cytometry Core Facility offers:
- cell sorting service
- analytical cytometry
- metabolic measurements
- individualized support for experiment planning and troubleshooting
The BD FACS AriaIII and FACS Fusion cell sorters are operated exclusively by the FCCF staff.
Users with no experience using FACS analyzers will need to be trained by one of the FCCF staff and will need to reach a level of competence allowing independent handling and operation of the analyzers.
Acknowledgements and Instrument-specific information
Acknowledging the FCCF staff and listing the FCCF equipment you used in the Materials and Methods section helps us to demonstrate how much our Facility contributes to the academic community and track the impact of our work and infrastructure. For acknowledgements, please use the template text provided below. If a FCCF staff member has made significant contributions to the progress of your project, e.g. by introducing a new idea or improving the approach, please consider a co-authorship.
Analysers:
• Fortessa: Samples were acquired on a BD LSRFortessa flow cytometer equipped with 4 lasers (405nm, 488nm, 561nm, and 640nm) and 16 fluorescence detectors.
• Cytek Aurora: Samples were acquired on a Cytek Aurora flow cytometer equipped with 5 lasers (355nm, 405nm, 488nm, 561nm, and 640nm) and 64 fluorescence detectors.
• Symphony: Samples were acquired on a BD FACSymphony A5 SE flow cytometer, equipped with 5 lasers (355nm, 405 nm, 488 nm, 561 nm, 640 nm) and 48 fluorescence detectors.
• Accuri: Samples were acquired on a BD Accuri C6 Plus flow cytometer, equipped with 2 lasers (488nm and 633nm), 2 light scatter detectors and 4 fluorescence detectors.
Cell Sorters:
• FACSAria Fusion: Samples were sorted on a BD FACSAria Fusion equipped with 5 lasers (355nm, 405nm, 488nm, 561nm, and 633nm) and 18 fluorescence detectors, using a 70/85/100/130um nozzle and standard pressure setup.
• FACSAriaIII: Samples were sorted on a BD FACSAria III running FACSDiva, equipped with 405nm (375nm), 488nm, 561 and 633nm lasers, with 16 fluorescence detectors, using a 70/85/100/130um nozzle and standard pressure setup.
Other:
• Seahorse: Samples were acquired on an Agilent Seahorse XFe96 Analyser
• Cytation: Cells were counted using an Agilent BioTek Cytation 1 cell imaging multimode reader.
• Miltenyi GentleMACS Octo Dissociator: Tissue samples were dissociated using a Miltenyi GentleMACS Octo following the manufacturer’s instructions.
Screening@ETH
Along with the other techonology platforms, such as NEXUS, GEML, Scope M and LAF, FCCF is a part of the Screening@ETH virtual platform, which focuses on arrayed and pooled screening approaches with the goal to tailor screening campaigns to your individual needs.
For more information, please got to the following link: