About us
Our facility is located on the G level of the HPL building, room G25.2, Otto-Stern-Weg 7, ETH Zurich Hoenggerberg.
ETH Zurich Flow Cytometry Core Facility
ETH Zurich
HPL G25.2
Otto-Stern-Weg7
8093
Zürich
Switzerland
Cell sorting
Our sorters are available Monday to Friday, 9:30-17:30. Extended sorting times are possible by special arrangement.
Analysis
The analysers are available 24/7 for independent users.
The building is open from 7:00 to 19:00, if you need to use the analysers outside of these hours, please arrange access to the HPL building on your own.
Analysis with support/ Introductions: Monday to Friday, 9:00-17:30
Supported analysis is on demand only, please contact us at least one day in advance to make sure somebody will be available to assist you.
24/7 for independent users
Acknowledging the FCCF staff and listing the FCCF equipment you used in the Materials and Methods section helps us to demonstrate how much our Facility contributes to the academic community and track the impact of our work and infrastructure. For acknowledgements, please use the template text provided below. If a FCCF staff member has made significant contributions to the progress of your project, e.g. by introducing a new idea or improving the approach, please consider a co-authorship.
Analysers:
• Fortessa: Samples were acquired on a BD LSRFortessa flow cytometer equipped with 4 lasers (405nm, 488nm, 561nm, and 640nm) and 16 fluorescence detectors.
• Cytek Aurora: Samples were acquired on a Cytek Aurora flow cytometer equipped with 5 lasers (355nm, 405nm, 488nm, 561nm, and 640nm) and 64 fluorescence detectors.
• Symphony: Samples were acquired on a BD FACSymphony A5 SE flow cytometer, equipped with 5 lasers (355nm, 405 nm, 488 nm, 561 nm, 640 nm) and 48 fluorescence detectors.
• Accuri: Samples were acquired on a BD Accuri C6 Plus flow cytometer, equipped with 2 lasers (488nm and 633nm), 2 light scatter detectors and 4 fluorescence detectors.
Cell Sorters:
• FACSAria Fusion: Samples were sorted on a BD FACSAria Fusion equipped with 5 lasers (355nm, 405nm, 488nm, 561nm, and 633nm) and 18 fluorescence detectors, using a 70/85/100/130um nozzle and standard pressure setup.
• FACSAriaIII: Samples were sorted on a BD FACSAria III running FACSDiva, equipped with 405nm (375nm), 488nm, 561 and 633nm lasers, with 16 fluorescence detectors, using a 70/85/100/130um nozzle and standard pressure setup.
Other:
• Seahorse: Samples were acquired on an Agilent Seahorse XFe96 Analyser
• Cytation: Cells were counted using an Agilent BioTek Cytation 1 cell imaging multimode reader.
• Miltenyi GentleMACS Octo Dissociator: Tissue samples were dissociated using a Miltenyi GentleMACS Octo following the manufacturer’s instructions.