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Flow Cytometry Core Facility

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Sorting Registration

Sorting Registration

Personal information:

Name ^*

Email ^*

Phone number ^*

Group Leader and Lab Address ^*

Sample:

Sample Origin ^*

Murine Human Cell Line Primary Human Yeast Bacteria Other

If other, please specify

Sample type: cell size, tissue origin (blood, digested lung, cell line etc.) ^* Please make sure that you bring single cell suspension Please make sure that you bring single cell suspension

Project description (biological background, goal of the experiment, cell preparation protocol, downstream application) ^*

Description of your experiment (number of samples, cell concentration, sample volume...) ^*

Sorting:

Nozzle size ^*

70 85 100 130 I do not know

Fluorophores (markers) / Excitation / Emission i.e. APC (CD4) / 640 nm / 660 nm i.e. APC (CD4) / 640 nm / 660 nm

Collection method ^*

1.5 ml tubes 5 ml tubes 15 ml tubes plate slide I don't know yet

Please specify if necessary: i.e. 96-well plate i.e. 96-well plate

Target subsets ^* i.e. subset 1: GFP+ (20% in sample) / sort 100'000 i.e. subset 1: GFP+ (20% in sample) / sort 100'000

If available, please attach the sorting scheme

Do you have any experience with cell sorting/analysis? Do you have any experience with BD FACS Aria III or Fusion? ^*

Biosafety:

Do the samples need special handling and/or security? ^*

Yes No

If so, please specify

If the cells are from culture, are they transduced with a viral vector?

Yes No

If yes, when was the transduction done?

Does the sample contain any other known human or animal infectious agent(s) or recombinant DNA materials? ^*

Yes No

Decontamination protocol: Wiping with ethanol 70% will be sufficient for decontamination. If not sufficient, enter other decontamination methods here: The BL2 samples must be fixed or can be analyzed in our BL2 lab, which is operated solely by Flow Cytometry Core Facility staff. The BL2 samples must be fixed or can be analyzed in our BL2 lab, which is operated solely by Flow Cytometry Core Facility staff.

ECOGEN permit

Please read and accept ^*

A 48 hour cancellation notice is required to avoid charges being applied to your account. Failing to cancel your appointment at least two days before your scheduled sort will result in you being charged for the original hours reserved despite absence or tardiness. Unused booked time will be charged in full. The Swiss Federal Office for the Environment (FOEN) requires that you add the ETHZ Flow Cytometry Core Facility room number to your ECOGEN notification, if you are using our services. Please add: ETH Zurich, HPL G25.2 for BL1 activities ETH Zurich, HPL G25.1 for BL2 activities

I would like to receive a copy of the form content by email.

Email

Note: The data will be sent unencrypted via Internet and may possibly be read by third parties.

^* Fields marked with an asterisk are mandatory.

Please check your information:

Sorting Project Confirmation

Thank you for submitting your project. Please register at our booking system: https://ezbooking.ethz.ch. We will contact you as soon as your registration is activated.

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